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KMID : 0624620220550030111
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BMB Reports
2022 Volume.55 No. 3 p.111 ~ p.112
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Gold and silver plasmonic nanoprobes trace the positions of histone codes
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Choi In-Hee
Song Ji-Hwan
Park Hyun-Sung
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Abstract
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We visualized the distribution of heterochromatin in a single nucleus using plasmonic nanoparticle-conjugated H3K9me3 and H3K27me3 antibodies. Due to distance-dependent plasmonic coupling effects between nanoprobes, their scattering spectra shift to longer wavelengths as the distance between heterochromatin histone markers reduced during oncogene-induced senescence (OIS). These observations were supported by simulating scattering profiles based on considerations of particle numbers, interparticle distances, and the spatial arrangements of plasmonic nanoprobes. Using this plasmon-based colourimetric imaging, we estimated changes in distances between H3K9me3 and H3K27me3 during the formation of senescence-associated heterochromatin foci in OIS cells. We anticipate that the devised analytical technique combined with high-spatial imaging and spectral simulation will eventually lead to a new means of diagnosing and monitoring disease progression and cellular senescence.
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KEYWORD
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Heterochromatin, Histone modification, Oncogene-induced senescence, Plasmonic coupling effect, Plasmonic nanoparticles, Single-cell imaging
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